|
KMID : 0620920080400040453
|
|
Experimental & Molecular Medicine
2008 Volume.40 No. 4 p.453 ~ p.460
|
|
|
Expression patterns of βig-h3 in chondrocyte differentiation during endochondral ossification
|
|
Han Min-Su
Shin Hong-In
Kim Jung-Eun
Kim In-San
|
|
|
Abstract
|
|
|
|
βig-h3 is a TGF-β-induced extracellular matrix protein which is expressed in many tissues including bones and cartilages. In previous reports, we showed that βig-h3 mediates cell adhesion and migration and, especially in bones, negatively regulates the mineralization in the end stage of endochondral ossification. Here, to elucidate the expression pattern and role of βig-h3 in chondrocyte differentiation, ATDC5 chondrocytes and embryonic and postnatal mice were used for in vitro differentiation studies and in vivo studies, respectively. βig-h3 was strongly induced by the treatment of TGF-β1 and the expression level of βig-h3 mRNA and protein were highly expressed in the early stages of differentiation but decreased in the late stages in ATDC5. Furthermore, the patterns of TGF-β1, -β2, and -β3 mRNA expression were concurrent with βig-h3 in ATDC5. βig-h3 was deeply stained in perichondrium (PC), periosteum (PO), and prehypertrophic chondrocytes (PH) through the entire period of endochondral ossification in mice. βig-h3 was mainly expressed in PC and PH at embryonic days and obviously in PH in postnatal days. These results suggest that βig-h3 may play a critical role as a regulator of chondrogenic differentiation in endochondral ossification.
|
|
|
KEYWORD
|
|
|
cell differentiation, chondrocytes, osteogenesis, transforming growth factor-β
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
    
|
|